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sox2
Sox2, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sox2/Sox2+Antibody%2C+anti-human%2Fmouse%2C+REAfinity/pmc13352860-131-60-63
Average 93 stars, based on 20 article reviews
Sox2, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sox2/Sox2+Antibody%2C+anti-human%2Fmouse%2C+REAfinity/pmc13352860-131-60-63
Average 93 stars, based on 20 article reviews
sox2 - by Bioz Stars,
2026-09
93/100 stars
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1) Product Images from "Activation of the G-protein coupled estrogen receptor 1 (GPER1) reduces transient receptor potential vanilloid 1 (TRPV1) activity and human iPSC-derived nociceptive neuron firing"
Article Title: Activation of the G-protein coupled estrogen receptor 1 (GPER1) reduces transient receptor potential vanilloid 1 (TRPV1) activity and human iPSC-derived nociceptive neuron firing
Journal: Stem Cell Research & Therapy
doi: 10.1186/s13287-026-05174-3
Figure Legend Snippet: Reprogramming of adult human dermal fibroblasts (HDFa) into induced pluripotent stem cell (iPSC) line BO-VC1. Reprogramming was performed using the Epi5 ™ Episomal iPSC Reprogramming Kit, enabling the generation of ( A ) transgene- and virus-free iPSC line BO-VC1. Successful generation of fibroblast-derived iPSCs was validated by immunocytochemical staining for the pluripotency markers ( B ) SOX2, C TRA 1–60, D OCT4, SSEA4 and E NANOG. Quantification of the generated iPSCs via flow cytometry revealed F 99.31% SSEA4/TRA1-60, G 97.43% SOX2/TRA1-60 and H 98.60% OCT3/4/TRA1-60 positive cells. I Quantification of the transcript levels of the stem cell markers NANOG , OCT4 , REX1 and SOX2 revealed significantly higher mRNA levels in iPSC line BO-VC1 compared to the HDFa control ( n = 3 different passages) Moreover, generated iPSCs were functionally validated by directed differentiation into all three germ layers. Subsequent immunocytochemical staining of the iPSCs before and after differentiation confirmed the expression of ( J–M ) meso-, N–Q endo- and R–U ectodermal lineage markers only in the respective differentiations. Scale bars: 50 μm. Data were tested for normal distribution using Shapiro-Wilk test. Means ± SEM (standard error of the mean) were statistically analyzed by a Kruskal-Wallis test with Dunn’s multiple comparisons test. n = 3 (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001)
Techniques Used: Virus, Derivative Assay, Staining, Generated, Flow Cytometry, Control, Expressing
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Article Snippet: For surface marker analyses, diluted or spiked samples were fixed by 4% methanol-free paraformaldehyde (PFA; Thermo Fisher Scientific) at RT for 10 min, blocked by 1% BSA (Sigma-Aldrich) in PBS (Wisent Bioproducts, Canada) on ice for 30 min, and stained by antibodies of SSEA-1, SSEA-4, TRA-1-60, TRA-1-81, CD324 (E-cadherin), CD326 (EpCAM), CD9, or CD90 (all from Miltenyi Biotec, Germany) for 10 min at 4°C in a flow buffer containing 1% BSA in PBS. .. For intracellular marker analyses, samples were fixed by 4% PFA at RT for 10 min, permeabilized by 0.5% Triton X-100 (Sigma-Aldrich) in PBS at RT for 10 min, blocked by 1% BSA in PBS on ice for 30 min, and stained by antibodies of Article Title: Activation of the G-protein coupled estrogen receptor 1 (GPER1) reduces transient receptor potential vanilloid 1 (TRPV1) activity and human iPSC-derived nociceptive neuron firing Article Snippet: After harvesting, using Accutase, 1 × 10 6 cells were stained for the surface stem cell marker TRA1-60 (1:50, #130-122-965, Miltenyi Biotec, Bergisch Gladbach, Germany) and SSEA4 (1:50, #130-124-073, Miltenyi Biotec, Bergisch Gladbach, Germany) in 25 μl PEB buffer (PBS + 0.5% BSA) for 10 min at 4 °C, washed in 500 μl PEB buffer and centrifuged at 200xg for 5 min. .. Subsequently, cells were incubated in inside fix solution of the Inside Stain Kit (#130-090-477, Miltenyi Biotec, Bergisch Gladbach, Germany) for 20 min at RT and centrifuged at 200xg for 5 min. After washing in 500 μl PEB buffer and centrifugation at 200xg cells were stained with the nuclear stem cell markers OCT3/4 (1:50, #130-117-821, Miltenyi Biotec, Bergisch Gladbach, Germany) and Blocking Assay:Article Title: Rejuvenated human amniotic fluid stem cells: a superior source of standardized induced mesenchymal stem cells for enhanced therapeutic applications Article Snippet: The cells were washed in PBS, fixed in 4% paraformaldehyde (PFA, Merck) and permeabilized using Triton X-100 (Merck). .. The cells were then blocked for 30 minutes with blocking buffer (PBS supplemented with 2% bovine serum albumin and 0.1% Tween) and incubated overnight with primary antibodies at their optimal dilution, i.e. OCT4A (Santa Cruz), KLF4 (Stemgent), Incubation:Article Title: Rejuvenated human amniotic fluid stem cells: a superior source of standardized induced mesenchymal stem cells for enhanced therapeutic applications Article Snippet: The cells were washed in PBS, fixed in 4% paraformaldehyde (PFA, Merck) and permeabilized using Triton X-100 (Merck). .. 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